| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sands, Samantha B (12)
|
ADCPP |
33.42 |
33.64 |
0.22 |
| 2 |
Ramgulam, Varshana (12)
|
ADCPP |
32.64 |
33.80 |
1.16 |
| 3 |
Stoup, Anna L (12)
|
ADCPP |
34.52 |
34.26 |
-0.26 |
| 4 |
Michels, Delaney R (12)
|
ADCPP |
33.33 |
34.89 |
1.56 |
| 5 |
Zhang, Stella Y (12)
|
ADCPP |
35.88 |
36.37 |
0.49 |
| 6 |
Bieniek, Allison M (12)
|
ADCPP |
35.59 |
37.08 |
1.49 |
| 7 |
Xue, Nichole Y (12)
|
ADCPP |
35.05 |
37.50 |
2.45 |
| 8 |
Guha, Mahika (12)
|
ADCPP |
37.67 |
39.78 |
2.11 |
| 9 |
Silaika, Genevieve E (11)
|
ADCPP |
38.41 |
39.92 |
1.51 |
| 10 |
Ensminger, Annabelle G (11)
|
ADCPP |
45.04 |
48.07 |
3.03 |
| 11 |
Joshi, Aishi (12)
|
ADCPP |
49.90 |
51.40 |
1.50 |
| 12 |
Klingbeil, Fiona P (12)
|
ADCPP |
|
56.87 |
- |